Glutaraldehyde cross-linked chitosan microspheres as a long acting biodegradable drug delivery vehicle: studies on the in vitro release of mitoxantrone and in vivo degradation of microspheres in rat muscle

摘要: Chitosan microspheres were prepared from 74% deacetylated chitin by the glutaraldehyde cross-linking of an aqueous acetic acid dispersion chitosan in a mixture liquid paraffin and petroleum ether stabilized using sorbitan sesquioleate as surfactant. Cross-linking hardening spherical particles achieved addition glutaraldehyde-saturated toluene through organic phase. A relatively novel antineoplastic agent, mitoxantrone, was incorporated into drug release studied vitro phosphate buffer for over 4 weeks at 27 degrees C. Drug found to be effectively controlled extent cross-linking. Only about 25% released 36 days high density. Implantation placebo skeletal muscle rats carried out order assess biocompatibility biodegradability microspheres. Histological analysis showed that well tolerated living tissue. However, no significant biodegradation material noticed period 3 months rats. Data obtained indicate possibility cross-linked carrier sustained very long periods.