The use of local anaesthetic microinjections to identify central pathways: a quantitative evaluation of the time course and extent of the neuronal block.
作者： J. Sandk�hler , B. Maisch , M. Zimmermann
DOI: 10.1007/BF00255242
关键词: Microinjections 、 Lumbar Spinal Cord 、 Spinal cord 、 Pipette 、 Lidocaine 、 Cord 、 Chemistry 、 Sagittal plane 、 Anesthesia 、 Stimulation
摘要: The time course and extent of local anaesthetic blocks within the spinal cord cats were evaluated. A monopolar stimulation electrode with tip lowered into dorsal columns (DC) 1000 μm below surface was used to activate antidromically DC fibers at T13 level evoke dorsum potentials lumbar cord. amplitude negative deflection, N-wave, determined for various intensities (stimulation-response-function, SRF). Lidocaine (1%) microinjected in volumes 0.5 or 1.0 μl from a glass micropipette 1 mm caudal site. Conduction block characterized by reversible shift SRFs higher intensities. diameter blocked area transverse plane evaluated threshold found be 0.9±0.1 4 30 min after injection lidocaine 1.6±0.36 10 45 lidocaine. In sagittal plane, following up 2.8 mm. DC-block 92 69 application present findings other CNS structures is discussed.
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sci-hub.se 参考文章(51)
J. Sandkuhler, Q. G. Fu, M. Zimmermann, Spinal pathways mediating tonic or stimulation-produced descending inhibition from the periaqueductal gray or nucleus raphe magnus are separate in the cat Journal of Neurophysiology. ,vol. 58, pp. 327- 341 ,(1987) , 10.1152/JN.1987.58.2.327
G. F. Gebhart, J. Sandkuhler, J. G. Thalhammer, M. Zimmermann, Inhibition of spinal nociceptive information by stimulation in midbrain of the cat is blocked by lidocaine microinjected in nucleus raphe magnus and medullary reticular formation Journal of Neurophysiology. ,vol. 50, pp. 1446- 1459 ,(1983) , 10.1152/JN.1983.50.6.1446
IA Iakhnitsa, NV Bulgakova, AI Piliavskiĭ, None, Effect of drugs potentiating or inhibiting the activity of locus coeruleus neurons on various types of spinal inhibition in the cat Neir̆ofiziologiia. ,vol. 13, pp. 247- 258 ,(1981)
Cairns Am, Fink Br, Differential peripheral axon block with lidocaine: unit studies in the cervical vagus nerve. Anesthesiology. ,vol. 59, pp. 182- ,(1983)
P. B. Brown, L. A. Ritz, Dorsal horn potentials and current source densities evoked by single action potentials in single slowly adapting type I axons. Journal of Neurophysiology. ,vol. 55, pp. 1104- 1113 ,(1986) , 10.1152/JN.1986.55.5.1104
GAYLORD D. ELLISON, The Use of Microknives in Brain Lesion Studies and the Production of Isolated Brain-stem Islands Methods in Psychobiology#R##N#Specialized Laboratory Techniques in Neuropsychology and Neurobiology. pp. 303- 318 ,(1972) , 10.1016/B978-0-12-512302-0.50015-8
A Staiman, P Seeman, Conduction-blocking concentrations of anesthetics increase with nerve axon diameter: studies with alcohol, lidocaine and tetrodotoxin on single myelinated fibers. Journal of Pharmacology and Experimental Therapeutics. ,vol. 201, pp. 340- 349 ,(1977)
A S Paintal, Block of conduction in mammalian myelinated nerve fibres by low temperatures. The Journal of Physiology. ,vol. 180, pp. 1- 19 ,(1965) , 10.1113/JPHYSIOL.1965.SP007686
A. G. Brown, Organization in the Spinal Cord: The Anatomy and Physiology of Identified Neurones ,(2011)
LUKE M KITAHATA, ARTHUR TAUB, ISAO SATO, None, Lamina-specific suppression of dorsal horn unit activity by nitrous oxide and by hyperventilation. Journal of Pharmacology and Experimental Therapeutics. ,vol. 176, pp. 101- 108 ,(1971)