Physiology of Rhodopsin and Iodopsin. Electromagnetic Conception for the Eyesight in Additive Mixing of Colors

作者: Ignat Ignatov , Oleg Mosin

DOI:

关键词: PigmentAdditive colorColor visionBacteriorhodopsinSize-exclusion chromatographyRhodopsinVisible spectrumHalobacteriumAnalytical chemistryChemistry

摘要: This review article views predominately the structure and function of animal bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) their aspects nano- biotechnological usage. On an example bacteriorhodopsin is described method its isolation from purple membranes photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis distilled water, processing biomass ultrasound at 22 KHz, alcohol extraction low high-weight molecular impurities, RNA, carotenoids lipids, solubilization with 0,5 % (w/v) SDS-Na subsequent fractionation methanol gel filtration chromatography on Sephadex G-200 Column balanced 0.09 M Tris-borate buffer ( pH = 8,35) 0,1 2,5 mM EDTA. Within framework research mechanism color perception visual analyzer having ability to analyze certain ranges optical spectrum, as colors was studied along analysis additive mixing two colors. It shown that electromagnetic waves different wavelengths, perceive them separate or average wave length corresponding mix color. Keywords : vision, rhodopsin, bacteriorhodopsin,

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