作者: Ignat Ignatov , Oleg Mosin
DOI:
关键词: Pigment 、 Additive color 、 Color vision 、 Bacteriorhodopsin 、 Size-exclusion chromatography 、 Rhodopsin 、 Visible spectrum 、 Halobacterium 、 Analytical chemistry 、 Chemistry
摘要: This review article views predominately the structure and function of animal bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) their aspects nano- biotechnological usage. On an example bacteriorhodopsin is described method its isolation from purple membranes photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis distilled water, processing biomass ultrasound at 22 KHz, alcohol extraction low high-weight molecular impurities, RNA, carotenoids lipids, solubilization with 0,5 % (w/v) SDS-Na subsequent fractionation methanol gel filtration chromatography on Sephadex G-200 Column balanced 0.09 M Tris-borate buffer ( pH = 8,35) 0,1 2,5 mM EDTA. Within framework research mechanism color perception visual analyzer having ability to analyze certain ranges optical spectrum, as colors was studied along analysis additive mixing two colors. It shown that electromagnetic waves different wavelengths, perceive them separate or average wave length corresponding mix color. Keywords : vision, rhodopsin, bacteriorhodopsin,