Analysis of function and regulation of proteins that mediate signal transduction by use of lipid-modified plasma membrane-targeting sequences.

摘要: It is now established that the function of many signaling molecules controlled, in part, by regulation subcellular localization. For example, dynamic recruitment normally cytosolic proteins to plasma membrane, activated Ras or receptor tyrosine kinases, facilitates their interaction with other membrane-associated components participate full activation (e.g., Raf-1). Therefore, creation chimeric contain lipid-modified sequences direct membrane localization allows generation constitutively variants such proteins. The amino-terminal myristoylation signal sequence Src family and carboxy-terminal prenylation have been widely used achieve this goal. Such membrane-targeted proved be valuable reagents study biochemical biological properties molecules.