Dual inhibition of the epidermal growth factor and vascular endothelial growth factor phosphorylation for antivascular therapy of human prostate cancer in the prostate of nude mice

摘要: BACKGROUND Androgen-independent prostate cancer (PCa) may be susceptible to modulation of the tumor microenvironment. We determined whether a dual tyrosine kinase inhibitor (AEE788) epidermal growth factor receptor (EGF-R) and vascular endothelial (VEGF-R) combined with chemotherapy can produce therapy human PCa in nude mice. METHODS PC-3MM2 cells were injected into mice. Three days later, mice randomized four groups: saline control, paclitaxel, AEE788, AEE788 paclitaxel. The treated for 5 weeks necropsied. Tumor incidence, weight, incidence lymph node metastasis recorded. tissue was analyzed immunohistochemically. RESULTS Treatment or plus paclitaxel significantly decreased total metastasis. treatment alone combination inhibited phosphorylation EGF-R VEGF-R on tumor-associated cells. Therapeutic efficacy correlated an increase apoptosis cells. CONCLUSION Blockade signaling pathways coupled suppressed progressive growing orthotopically © 2005 Wiley-Liss, Inc.