Proper functioning of the GINS complex is important for the fidelity of DNA replication in yeast

摘要: Summary The role of replicative DNA polymerases in ensuring genome stability is intensively studied, but the other components replisome still not fully understood. One such component GINS complex (comprising Psf1, Psf2, Psf3 and Sld5 subunits), which participates both initiation elongation replication. Until now, understanding physiological mostly originated from biochemical studies. In this article, we present genetic evidence for an essential maintenance replication fidelity Saccharomyces cerevisiae. our studies employed psf1-1 allele (Takayama et al., 2003) a novel psf1-100 isolated laboratory. Analysis levels specificity mutations psf1 strains indicates that destabilization or its impaired interaction with polymerase epsilon increases level spontaneous mutagenesis participation error-prone zeta. Additionally, synergistic mutator effect was found defects Psf1p proofreading activity Pol epsilon, suggesting proper functioning crucial facilitating error-free processing terminal mismatches created by epsilon.