Single-chain antibody streptavidin fusions: Tetrameric bifunctional scFv-complexes with biotin binding activity and enhanced affinity to antigen
作者： Sergey M. Kipriyanov , Frank Breitling , Melvyn Little , Stefan Dübel
关键词: Streptavidin 、 Avidity 、 Chemistry 、 Biotinylation 、 Biotin binding 、 Affinity chromatography 、 Biotin 、 Molecular biology 、 Fusion protein 、 Binding site
摘要: … COli23• By fusing scFv DNA with this core-streptavidin gene, we have been able to form tetrameric antibodies. In addition, the pSTE vector introduces a cysteine close to the C-terminus …
sci-hub.se 参考文章(2)
R. Bird, K. Hardman, J. Jacobson, S Johnson, B. Kaufman, S. Lee, T Lee, S. Pope, G. Riordan, M Whitlow, Single-chain antigen-binding proteins Science. ,vol. 242, pp. 423- 426 ,(1988) , 10.1126/SCIENCE.3140379
R. Crea, J. S. Huston, D. Levinson, M. Mudgett-Hunter, M. S. Tai, J. Novotny, M. N. Margolies, R. J. Ridge, R. E. Bruccoleri, E. Haber, Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 5879- 5883 ,(1988) , 10.1073/PNAS.85.16.5879