A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway

摘要: Wounding plant tissues initiates large-scale changes in transcription coupled to growth arrest, allowing resource diversion for defense. These processes are mediated large part by the potent lipid regulator jasmonic acid (JA). Genes selected from a list of wound-inducible transcripts regulated jasmonate pathway were overexpressed Arabidopsis thaliana, and transgenic plants then assayed sensitivity methyl (MeJA). When grown presence MeJA, roots overexpressing gene unknown function longer than those wild-type plants. transcript levels this gene, which we named JASMONATE-ASSOCIATED1 (JAS1), reduced RNA interference, showed increased MeJA was inhibited. gain- loss-of-function assays suggest that acts as repressor JA-inhibited growth. An alternative encoding second protein isoform with C terminus failed repress sensitivity. This identified conserved C-terminal sequence JAS1 related genes, all also contain Zim motifs many jasmonate-regulated. Both forms found localize nucleus transient expression assays. Physiological tests responses after wounding consistent fact is JA-regulated retardation.