A high-throughput approach for measuring temporal changes in the interactome
作者： Anders R Kristensen , Joerg Gsponer , Leonard J Foster
DOI: 10.1038/NMETH.2131
关键词: Biochemistry 、 Proteins metabolism 、 Interactome 、 Temporal information 、 Computational biology 、 Protein Interaction Map 、 Throughput (business) 、 Proteomics 、 Biology 、 High-Throughput Screening Assays 、 Quantitative proteomics
摘要: Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome the same depth accuracy as AP-MS with less work without overexpression tagging. The use triplex labeling enables monitoring rearrangements.
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