Comparative Analysis of Complete Chloroplast Genome Sequences of Wild and Cultivated Bougainvillea (Nyctaginaceae).

摘要: Bougainvillea (Nyctaginaceae) is a popular ornamental plant group primarily grown for its striking colorful bracts. However, despite established horticultural value, limited genomic resources and molecular studies have been reported this genus. Thus, to address existing gap, complete chloroplast genomes of four species (Bougainvillea glabra, peruviana, pachyphylla, praecox) one cultivar were sequenced characterized. The cp range from 153,966 bp 154,541 in length, comprising large single-copy region (85,159 bp–85,708 bp) small (18,014 bp–18,078 separated by pair inverted repeats (25,377–25,427 bp). All plastomes 131 annotated genes, including 86 protein-coding, eight rRNA, 37 tRNA genes. These five newly compared the spectabilis genome deposited GeBank. results showed that all highly similar structures, contents, organization. They exhibit quadripartite structures same numbers genes introns. Codon usage, RNA editing sites, repeat analyses also revealed six genomes. amino acid leucine has highest proportion almost favored synonymous codons either an A or U ending. Likewise, out 42 predicted most conversions serine (S) (L). majority simple sequence detected A/T mononucleotides, making A/T-rich. contractions expansions IR boundaries very minimal as well, hence contributing little differences size. In addition, variation share nearly identical profiles though several potential barcodes, such ycf1, ndhF, rpoA identified. Higher was observed both B. peruviana pachyphylla sequences based on SNPs indels analysis. Phylogenetic reconstructions further these two appear be basal taxa Bougainvillea. rarely cultivated wild (B. diverged earlier than commonly spectabilis, cv.). Overall, study provide additional genetic can aid phylogenetic evolutionary Moreover, information potentially useful identifying cultivars, which essential taxonomic breeding studies.