Isolation and pharmacological characterisation of hostoxin-1, a postsynaptic neurotoxin from the venom of the Stephen's banded snake (Hoplocephalus stephensi).
作者： A SMITH , S REEVE , W HODGSON , L TAN , S KURUPPU
DOI: 10.1016/J.NEUROPHARM.2006.05.020
关键词: Venom 、 Snake venom 、 Neurotoxin 、 Postsynaptic potential 、 Antivenom 、 Nicotinic agonist 、 Pharmacology 、 Acetylcholine 、 Anesthesia 、 Biology 、 Carbachol
摘要: Envenoming by the Stephen's banded snake (Hoplocephalus stephensi) is not usually characterised neurotoxicity. The present study describes pharmacological characterisation of hostoxin-1 (MW 6660 Da), first neurotoxin to be isolated from venom snake. Hostoxin-1 (0.3-1.0 microM) caused concentration-dependent inhibition indirect twitches chick biventer cervicis nerve-muscle preparation. neurotoxic activity (0.3 was irreversible washing, but significantly reversed addition CSL tiger antivenom (5 units/ml) added at t90 (i.e. time which were inhibited 90%). In addition, responses exogenous acetylcholine and carbachol, KCl, indicating a postsynaptic mode action. (5-30 nM) displayed pseudo-irreversible antagonism skeletal muscle nicotinic receptor with pA2 value 8.45+/-0.32 approximately 100-fold more potent than tubocurarine). H. stephensi high level PLA2 (specific 100.1+/-4.4 micromol/min/mg). However, negligible. Partial N-terminal sequencing indicates that it has sequence homology other elapid short-chain neurotoxins.
elsevier.com
sci-hub.se 参考文章(20)
H S Kim, N Tamiya, Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) Biochemical Journal. ,vol. 193, pp. 899- 906 ,(1981) , 10.1042/BJ1930899
Struan K. Sutherland, Australian animal toxins: The creatures, their toxins, and care of the poisoned patient ,(1983)
Gregory P Comadira, David Green, Three cases of envenomation by Stephen's banded snakes Emergency Medicine Australasia. ,vol. 7, pp. 15- 18 ,(2009) , 10.1111/J.1442-2026.1995.TB00186.X
A.J. Broad, S.K. Sutherland, A.R. Coulter, The lethality in mice of dangerous Australian and other snake venom Toxicon. ,vol. 17, pp. 661- 664 ,(1979) , 10.1016/0041-0101(79)90245-9
Arthur Christopoulos, Peter Coles, Lucy Lay, Michael J Lew, James A Angus, Pharmacological analysis of cannabinoid receptor activity in the rat vas deferens British Journal of Pharmacology. ,vol. 132, pp. 1281- 1291 ,(2001) , 10.1038/SJ.BJP.0703930
Janith C Wickramaratna, Bryan G Fry, Richard E Loiacono, Marie-Isabel Aguilar, Paul F Alewood, Wayne C Hodgson, Isolation and characterization at cholinergic nicotinic receptors of a neurotoxin from the venom of the Acanthophis sp. Seram death adder. Biochemical Pharmacology. ,vol. 68, pp. 383- 394 ,(2004) , 10.1016/J.BCP.2004.03.033
Selvanayagam Nirthanan, Eric Charpantier, Ponnampalam Gopalakrishnakone, Matthew C. E. Gwee, Hoon-Eng Khoo, Li-Sam Cheah, Daniel Bertrand, R. Manjunatha Kini, Candoxin, a Novel Toxin fromBungarus candidus, Is a Reversible Antagonist of Muscle (αβγδ) but a Poorly Reversible Antagonist of Neuronal α7 Nicotinic Acetylcholine Receptors Journal of Biological Chemistry. ,vol. 277, pp. 17811- 17820 ,(2002) , 10.1074/JBC.M111152200
Bryan Grieg Fry, Structure–function properties of venom components from Australian elapids Toxicon. ,vol. 37, pp. 11- 32 ,(1999) , 10.1016/S0041-0101(98)00125-1
A.L. Harvey, A. Barfaraz, E. Thomson, A. Faiz, S. Preston, J.B. Harris, Screening of snake venoms for neurotoxic and myotoxic effects using simple in vitro preparations from rodents and chicks Toxicon. ,vol. 32, pp. 257- 265 ,(1994) , 10.1016/0041-0101(94)90078-7
Veena S. RAO, Jeremiah S. JOSEPH, R. Manjunatha KINI, Group D prothrombin activators from snake venom are structural homologues of mammalian blood coagulation factor Xa. Biochemical Journal. ,vol. 369, pp. 635- 642 ,(2003) , 10.1042/BJ20020889