Entry of Tobacco Mosaic Virus RNA into Tobacco Chloroplasts in Vivo and in Vitro

摘要: Chloroplasts may be adversely affected in tobacco mosaic virus (TMV)-infected plants. Part of the perturbation could result from known accumulation and viral-coded components within that organelle. Our studies have been directed at ascertaining how these virus-related gain entry into chloroplasts. We prepared chloroplasts either directly-inoculated or systemically-infected leaves TMV-infected tobacco, uninfected controls. Intact chloroplasts, isolated Percoll gradients, were treated with both pancreatic ribonuclease thermolysin to destroy any TMV particles RNA, bound copurifying RNA such was probed on Northern blots 32P-labelled cDNA RNA. Genomic, but not sub-genomic detected systemically-invaded leaves, indicating uptake chloroplast is selective. Evidence showing per se (as opposed virions) enter based observation temperature-sensitive mutant Ts 38, which no virions are formed a restrictive temperature 35°, still allows for viral temperature.