THE ROLE OF NITRIC OXIDE IN DIATOM ADHESION IN RELATION TO SUBSTRATUM PROPERTIES1
作者： Stephanie E. M. Thompson , Alison R. Taylor , Colin Brownlee , Maureen E. Callow , James A. Callow
DOI: 10.1111/J.1529-8817.2008.00531.X
关键词: Adhesion 、 Polydimethylsiloxane 、 Biochemistry 、 Nitric oxide synthase 、 Context (language use) 、 Extracellular polymeric substance 、 Biophysics 、 Biology 、 Biofouling 、 Nitric oxide 、 Biofilm
摘要: Adhesion of raphid diatoms to surfaces, mediated by the secretion extracellular polymeric substances (EPS), is an important strategy for growth and survival. Diatom biofilms are also in context biofouling. Diatoms exhibit selectivity adhering but little understood about how they perceive properties a substratum translate that perception into altered adhesion properties. In this study, we demonstrate Seminavis robusta Danielidis et D. G. Mann, like many other pennate diatoms, adheres more strongly hydrophobic surfaces (such as silicone elastomer foul-release coatings) than hydrophilic surfaces. To explore cellular mechanisms may underlie selectivity, tested hypothesis surface unconducive through form stress response involving nitric oxide (NO) production. Single-cell imaging with fluorescent indicator DAF-FM DA (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate), revealed NO levels were 4-fold higher cells adhered (acid-washed glass) compared (polydimethylsiloxane elastomer, PDMSE). Elevated caused addition donor S-nitroso-N-acetylpenicillamine (SNAP) did not affect growth, showed reduced strength both glass PDMSE. Addition synthase inhibitor NG-monomethyl-l-arginine (NMMA) small significant increase strength. Overall, results suggest acts signal wettability substrata Seminavis.
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