STI571 (Glivec) suppresses the expression of vascular endothelial growth factor in the gastrointestinal stromal tumor cell line, GIST-T1
作者： Toufeng Jin , Hajime Nakatani , Takahiro Taguchi , Takumi Nakano , Takehiro Okabayashi
关键词: Stem cell factor 、 Vascular endothelial growth factor A 、 Biology 、 Cell growth 、 Cancer research 、 Vascular endothelial growth factor 、 Endocrinology 、 Flow cytometry 、 Cell culture 、 Viability assay 、 Internal medicine 、 MTT assay 、 Gastroenterology 、 General Medicine
摘要: AIM: To estimate whether STI571 inhibits the expression of vascular endothelial growth factor (VEGF) in gastrointestinal stromal tumor (GIST) cells. METHODS: We used GIST cell line, GIST-T1. It has a heterogenic 57-bp deletion exon 11 to produce mutated c-KIT, which results constitutive activation c-KIT. Cells were treated with/without or stem (SCF). Transcription and VEGF determined by RT-PCR flow cytometry Western blotting, respectively. Activated c-KIT was estimated immunoprecipitation analysis. Cell viability MTT assay. RESULTS: Activation inhibited treatment. suppressed at both transcriptional translational levels temporal dose-dependent manner STI571. SCF upregulated it also reduced GIST-T1 cells, as assay. CONCLUSION: regulated antitumor effects on cells with respect not only inhibition growth. but suppression expression.
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