Generation of insulin-producing β-like cells from human iPS cells in a defined and completely xeno-free culture system.

摘要: Human induced pluripotent stem (hiPS) cells are considered a potential source for the generation of insulin-producing pancreatic β-cells because their differentiation capacity. In this study, we have developed five-step xeno-free culture system to efficiently differentiate hiPS into in vitro. We found that high NOGGIN concentration is crucial specifically inducing first and duodenal homeobox-1 (PDX1)-positive progenitors then neurogenin 3 (NGN3)-expressing endocrine progenitors, while suppressing hepatic or intestinal cells. also combination 3-isobutyl-1-methylxanthine (IBMX), exendin-4, nicotinamide was important insulin single-positive expressed various β-cell markers. Most notably, differentiated contained endogenous C-peptide pools were released response secretagogues levels glucose. Therefore, our results demonstrate feasibility generating hiPS-derived under conditions highlight treat patients with type 1 diabetes.