A precise mRNA quantification method using CE-based SSCP

摘要: Even though mRNA quantification provides significant information for biological analysis, current methods such as Northern blot analysis and real-time PCR are known to be laborious lacking in precision. In this study, we demonstrate a new precise method using CE based on SSCP (CE-SSCP) coupled with reverse transcription. samples could simply analyzed the directly transcript obtained from single reaction. This helps avoid considerable errors generated by series of tedious manual steps. Also, unlike PCR, transcripts can quantified CE-SSCP without further data estimation. Reproducibility accuracy was examined enhanced green fluorescent protein (eGFP) transcribed vitro. Specific transcription primer determined accurate eGFP total RNA recombinant Escherichia coli. Using elongation factor Tu an internal standard, it shown that sample-to-sample variation minimized. Expression kinetics at both level studied potential expression demonstrated comparison activity assay.