Agarose infiltration improves morphology of cryostat sections of lung

摘要: Background Optimal morphology and immunohistochemistry of the lung requires organ to remain fully distended. When is prepared for frozen sections, especially during stage cryoprotection, liquid fixatives leak out tends collapse. We sought develop a new technique improve sections while facilitating tissue handling immunoreactivity. Experimental design Human, fetal sheep adult rat lungs were distended with 1% low temperature melting agarose vessels perfused paraformaldehyde solution. Results The infiltration permitted easy tissue, maintained architecture cryoprotection. Agarose fixation provided excellent morphology, that was comparable paraffin-embedded tissue. structural preservation noticeable human remained well technique. Because mild good precise localization antigenic sites possible surfactant proteins SP-A, SP-C SP-D, cytokeratin, alveolar macrophage markers. Conclusions This procedure can special embedding protocols immunocytochemistry immunofluorescence.