Characterization of hepatitis B virus major surface antigen gene transcriptional regulatory elements in differentiated hepatoma cell lines.
作者： A K Raney , D R Milich , A McLachlan
DOI: 10.1128/JVI.63.9.3919-3925.1989
关键词: Transcriptional regulation 、 Response element 、 Gene expression 、 Enhancer 、 Gene 、 Antigen Gene 、 Regulatory sequence 、 Transcription factor 、 Biology 、 Molecular biology
摘要: The regulatory DNA sequence elements that control the expression of hepatitis B virus major surface antigen gene in hepatoblastoma cell line HepG2 were analyzed by using transient transfection assays. In this system, enhancer increases transcription from promoter approximately twofold. regulating are within a 200-nucleotide located immediately upstream initiation sites. consists an 85-nucleotide distal element which two- to fourfold and proximal 115 nucleotides is essential for transcriptional activity. shown bind factors present nuclear extracts, consistent with role demonstrated these sequences. sequences hepatocellular carcinoma lines PLC/PRF/5 Hep3B was also demonstrated, indicating similar regulation each differentiated hepatoma lines.
sci-hub.st 参考文章(43)
Arthur Zelent, Peter Price, Saul Karpen, Identification ofProtein-Binding Sites intheHepatitis B Virus Enhancer andCorePromoter Domains ,(1988)
CM Chang, KING-SONG Jeng, Ch-P Hu, SJ Lo, TSUNG-SHENG Su, LP Ting, CHENG-KUNG Chou, SH Han, E Pfaff, J Salfeld, None, Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line. The EMBO Journal. ,vol. 6, pp. 675- 680 ,(1987) , 10.1002/J.1460-2075.1987.TB04807.X
J Honigwachs, O Faktor, R Dikstein, Y Shaul, O Laub, Liver-specific expression of hepatitis B virus is determined by the combined action of the core gene promoter and the enhancer. Journal of Virology. ,vol. 63, pp. 919- 924 ,(1989) , 10.1128/JVI.63.2.919-924.1989
Y. Shaul, R. Ben-Levy, T. De-Medina, High affinity binding site for nuclear factor I next to the hepatitis B virus S gene promoter. The EMBO Journal. ,vol. 5, pp. 1967- 1971 ,(1986) , 10.1002/J.1460-2075.1986.TB04451.X
C Pourcel, A Louise, M Gervais, N Chenciner, M F Dubois, P Tiollais, Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA. Journal of Virology. ,vol. 42, pp. 100- 105 ,(1982) , 10.1128/JVI.42.1.100-105.1982
William S. Robinson, David A. Clayton, Richard L. Greenman, DNA of a Human Hepatitis B Virus Candidate Journal of Virology. ,vol. 14, pp. 384- 391 ,(1974) , 10.1128/JVI.14.2.384-391.1974
C Sureau, J W Eichberg, G B Hubbard, J L Romet-Lemonne, M Essex, A molecularly cloned hepatitis B virus produced in vitro is infectious in a chimpanzee. Journal of Virology. ,vol. 62, pp. 3064- 3067 ,(1988) , 10.1128/JVI.62.8.3064-3067.1988
S Kaneko, R H Miller, X-region-specific transcript in mammalian hepatitis B virus-infected liver. Journal of Virology. ,vol. 62, pp. 3979- 3984 ,(1988) , 10.1128/JVI.62.11.3979-3984.1988
R. Cattaneo, H. Will, H. Schaller, Hepatitis B virus transcription in the infected liver. The EMBO Journal. ,vol. 3, pp. 2191- 2196 ,(1984) , 10.1002/J.1460-2075.1984.TB02113.X
A McLachlan, D R Milich, A K Raney, M G Riggs, J L Hughes, J Sorge, F V Chisari, Expression of hepatitis B virus surface and core antigens: influences of pre-S and precore sequences. Journal of Virology. ,vol. 61, pp. 683- 692 ,(1987) , 10.1128/JVI.61.3.683-692.1987