Differential regulation of hsp70 genes in the freshwater key species Gammarus pulex (Crustacea, Amphipoda) exposed to thermal stress: effects of latitude and ontogeny.

摘要: Temperature is one of the main abiotic factors influencing distribution and abundance organisms. In Rhone River Valley, populations crustacean Gammarus pulex are distributed along a 5 °C thermal gradient from North to South valley. this present work, we investigated heat shock response G. according latitudinal (northern vs. southern populations) ontogeny (adults embryos early stages). We isolated two isoforms (one constitutive hsc70 inducible hsp70) proteins 70 (HSP70) quantitatively compared their amounts mRNA after shocks, using real-time PCR. Whereas (constitutive) gene did not vary between populations, significant effect population was observed on expression hsp70 (inducible) in adult specimens. The northern amphipods showed greater magnitude induction 2 °C lower onset temperature when population, suggesting that more affected by elevated than one. demonstrated may play crucial role persistence biogeographical patterns pulex, since it reflects natural species gradient. A differential regulation also ontogenetic stage, with switch life stages constitutively highly expressed adults. These findings demonstrate importance considering entire cycle better understand adaptive stress.