Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes (Bubalus bubalis) from Brazil
作者： A.A.R. Rodrigues , S.M. Gennari , D.M Aguiar , C. Sreekumar , D.E. Hill
DOI: 10.1016/J.VETPAR.2004.07.007
关键词: Pcr assay 、 Neospora caninum 、 Biology 、 Parasite hosting 、 Bubalus 、 Antibody 、 Water Buffaloes 、 Bioassay 、 Veterinary medicine 、 Sequence analysis 、 General Veterinary 、 Parasitology
摘要: Attempts were made to isolate Neospora caninum from naturally infected water buffaloes (Bubalus bubalis) Brazil. Brains six with indirect fluorescent antibodies (>1:100) N. used the parasite by bioassay in dogs and gerbils followed vitro culture. Shedding of Neospora-like oocysts was noticed fed brains three (isolate designation NcBrBuf-1, 2 4). Two more isolates (NcBrBuf-3 5) obtained culture previously two other buffaloes. The identity confirmed biological molecular methods. found be non-pathogenic gerbils. All five amplified gene 5 amplicons using Neospora-specific PCR assay. sequences fragments common toxoplasmatiid ITS-1 analyzed. dynamics oocyst production indicate that are natural intermediate hosts for caninum. This is first report isolation
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