Fluorine Magnetic Resonance in Biochemistry

摘要: Proton magnetic resonance (PMR) spectroscopy has proved to be an important tool in structural studies of biological macromolecules (Roberts and Jardetzky, 1970; Casy, 1971; Jardetzky Wade-Jardetzky, Dwek, 1973; James, 1975; Wuthrich, 1976; Thomas, 1976). The fields currently available can provide frequencies 360 MHz or higher, with the spectral dispersion under these conditions, PMR is often method choice for examining small proteins peptides solution. In situations it usually possible assign many signals observed specific amino acids thus have a number spectroscopic probes into structure molecule; we take “structure” this context include not only sequence information but also details conformational motions.