GENERATIVE CELL SPECIFIC 1 is essential for angiosperm fertilization
作者： Toshiyuki Mori , Haruko Kuroiwa , Tetsuya Higashiyama , Tsuneyoshi Kuroiwa
DOI: 10.1038/NCB1345
关键词: Embryo 、 Sperm 、 Gamete 、 Gametophyte 、 Double fertilization 、 Pollen tube 、 Biology 、 Pollen tube reception 、 Cell biology 、 Gametogenesis
摘要: The double fertilization process in angiosperms is based on the delivery of a pair sperm cells by pollen tube (the male gametophyte), which elongates towards an embryo sac female gametophyte) enclosing egg and central cell. Several studies have described mechanisms gametophyte interaction, also - from pollination to acceptance. However, gamete interaction are not fully understood. Cytological shown that gametes possess distinct cell-surface structures genes specific been detected cDNA libraries. Thus, isolated may offer clues understanding sperm-egg interaction. In this study, we identified novel protein, designated GCS1 (GENERATIVE CELL SPECIFIC 1), using generative Lilium longiflorum pollen. possesses carboxy-terminal transmembrane domain, homologues present various species, including non-angiosperms. Immunological assays indicate accumulated during late gametogenesis localized plasma membrane cells. addition, Arabidopsis thaliana mutant fail fuse, resulting sterility suggesting critical factor angiosperms.
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