Cytotoxic T Lymphocytes in Resistance to Tuberculosis

摘要: Recent experimental evidence has suggested T cells recognizing antigens in the context of both classical MHC class I and nonclassical I-like molecules contribute to protective responses against Mycobacterium tuberculosis (MTB) infection. Our aims were characterize types cells, explore basis communication between tubercle bacilli pathway host macrophage. A model system was developed using exogenously added ovalbumin as a surrogate antigen study presentation by MTB-infected macrophages. Viable, virulent MTB closely related mycobacterial species facilitated on CD8+ cytolytic that dependent upon cytosolic transport peptides, implying phagosome cell cytoplasm. soluble mimicked Listeria monocytogenes, which grows within cytoplasm, well its purified hemolysin. We have also characterized recognize nonpeptide presented CD1 molecules. CD1-restricted demonstrated lyse macrophages infected with divided into distinct subsets based surface phenotype (CD4-XD8- versus CD8+) cytotoxicity mechanism (Fas receptor-mediated granule exocytosis). functional consequence these two mechanisms observed while lysed macrophages, only those utilizing exocytosis able reduce viability intracellular MTB.