Dopamine D1 receptor-mediated intracellular responses in the hypothalamus after co-administration of caffeine with MDMA.

摘要: Markers of dopamine D(1) receptor activation were determined to elucidate intracellular mechanisms associated with the combined effects caffeine and 3,4 methylenedioxymethamphetamine (MDMA), reported previously produce increased toxicity, when compared either drug alone. Caffeine (10 mg/kg) MDMA (15 mg/kg) administered male Sprague Dawley rats alone in combination. One hour after administration, core body temperature phosphorylation -related markers, cAMP response element binding protein (CREB), c-AMP-regulated phosphoprotein 32 kDa (DARPP-32) expression immediate early gene cellular marker c-fos hypothalamus. Co-administration or treatment Pre-treatment antagonist SCH 23390 (1 mg/kg, i.p.), 30 min. prior produced a hypothermic that was unaffected by caffeine. p-CREB, p-DARPP-32 SCH-23390 attenuated changes p-DARPP c-fos. The results show an enhanced is but not agent suggestive synergistic actions convergent on signalling pathway. A dopamine-related synergy administration may have important use safety implications for recreational users.