Inhibition of somatotroph growth and growth hormone biosynthesis by activin in vitro
作者： Nils Billestrup , Consuelo González-Manchón , Ellen Potter , Wylie Vale
DOI: 10.1210/MEND-4-2-356
关键词: Biology 、 Growth hormone secretion 、 Endocrinology 、 Secretion 、 Peptide hormone 、 Internal medicine 、 Pituitary gland 、 Somatotropic cell 、 Somatropin 、 Cell growth 、 Hormone
摘要: Activin-A, a homodimeric protein composed of two inhibin βA-subunits, was first isolated from gonadal fluids based upon its ability to stimulate FSH secretion and biosynthesis, but also observed suppress GH secretion. The present report describes the effects activin on biosynthesis proliferation pituitary somatotrophs. In cells cultured in presence 0.7 nm for 3 days, decreased by 50% compared control value. Inhibition measured quantitative immunoprecipitation [35S]methionine-labeled cells, could be after 24 h treatment, maximal (70%) inhibition days. Activin inhibited basal as well GH-releasing factor (GRF)-, glucocorticoid-, thyroid hormone-stimulated biosynthesis. Inhibin, which is known reverse effect secretion, did not Treatment somatotrophs with a...
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