Construction of electrochemiluminescence sensing platform with in situ generated coreactant strategy for sensitive detection of prostate specific antigen
作者： Fang Hou , Xue-Bo Hu , Shu-Hui Ma , Jun-Tao Cao , Yan-Ming Liu
DOI: 10.1016/J.JELECHEM.2019.113817
关键词: Detection limit 、 Nanotechnology 、 Chemistry 、 Luminol 、 Linear range 、 Prostate-specific antigen 、 Electrochemiluminescence 、 Biomolecule 、 In situ 、 Glucose oxidase
摘要: Abstract A sandwich-type ECL platform was fabricated for sensitive detection of prostate specific antigen (PSA) by using AuNPs decorated MoS2 (MoS2-AuNPs) as electrode material and SiO2 nanoparticles labeled with secondary antibody (Ab2) glucose oxidase (Ab2-SiO2-GOD) signal probes. Owing to the excellent conductivity large surface area MoS2-AuNPs, fast electron transfer process immobilization numerous biomolecules can be achieved. The GOD probes realize in situ generation H2O2 coreactant amplify intensity luminol. developed immunosensor displayed a wide linear range 0.5 pg mL−1–10.0 ng mL−1 low limit 0.20 pg mL−1 PSA detection. also has been successfully applied analysis human serum samples. Furthermore, proposed strategy shows great application prospects determination other biomarkers biological
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