Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes.

摘要: An emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On other awareness pros and cons chemically synthesized compounds' extensive use leads a search metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from buckwheat honey sample presented wide spectrum activity against both Gram-positive Gram-negative pathogens. Recent investigation has confirmed P. MP1 (deposited at DDBJ/ENA/GenBank under accession WSQB00000000) produces proteinaceous, heat-stable compound(s) with maximum production obtained after 18 hours growth LB medium 37 °C continuous shaking 200 RPM. The highest was found 40% ammonium sulfate precipitate, high also remaining 50% 60% precipitates. Moderate is insensitive proteases or heat treatment, pathogenic bacteria included L. monocytogenes FSL - X1-0001 (strain 10403S), S. aureus L1 0030 E. coli O157: H7. Further studies, including de novo sequencing peptides by mass spectrometry, are progress.