Single-cell laser-capture microdissection and RNA amplification.

摘要: To permit long-term measurement of time-dependent changes in levels dialyzable drugs and transmitters the spinal intrathecal (IT) space unanesthetized rat, we developed a dialysis catheter for chronic placement. This was accomplished by constructing loop-flexible probe 9 cm length from PE-5 polyethylene tubing 4 membrane. loop inserted through an incision cisternal membrane passed to lumbar enlargement. The ends were then externalized on top head. For dialysis, external end connected syringe pump perfused with artificial cerebrospinal fluid (CSF) out flow collected. Using this system demonstrate: a) significant increase CSF concentrations amino acids after traumatic injury, b) permeability drug (methylprednisolone) blood-brain barrier into extracellular systemic delivery, c) effect IT injection N-methyl-D-aspartate secondary acid release, clearance sufentanyl delivery corresponding development thermal nociception. provides robust experimental tool studying concentration diffusible substances over extended postimplantation interval allows comparison these concurrently assessed behavioral indices.