Improvements in the Sensitivity of Time Resolved Fluorescence Energy Transfer Assays
作者： Scott G. Jones , Denise Y. Lee , Jane F. Wright , Christopher N. Jones , Michelle L. Teear
关键词: Lanthanide 、 Förster resonance energy transfer 、 Time-resolved fluorescence energy transfer 、 Time-resolved spectroscopy 、 Photochemistry 、 Fluorescence 、 Chemistry 、 Acceptor 、 Fluorescence in the life sciences 、 Fluorescence cross-correlation spectroscopy
摘要: The effect on fluorescence resonance energy transfer (FRET) of multiple labelling DNA oligonucleotides with donor lanthanide chelate and acceptor CyDye™ fluors has been investigated. It is shown that using a single Cy™ or Cy5 can increase sensitivity output. enhanced FRET observed in the label system utilised two different based assay formats to demonstrate advantages over steady state radiometric assay.
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