Effects of nutrient deprivation on Vibrio cholerae.

作者: R M Baker , F L Singleton , M A Hood

DOI: 10.1128/AEM.46.4.930-940.1983

关键词: Vibrio choleraeMicrocosmCell divisionBacteriaCell wallNutrientArtificial seawaterMicrobiologyBiologyViable countApplied Microbiology and BiotechnologyBiotechnologyFood scienceEcology (disciplines)

摘要: Abstract Environmental and clinical strains of Vibrio cholerae were exposed to nutrient-free artificial seawater filtered natural microcosms for selected time intervals examined changes in cell morphology number. Cells observed by transmission electron epifluorescence microscopy found undergo gross alterations with exposure. The vibroid cells decreased volume 85% developed into small coccoid forms surrounded remnant walls. initial number inoculated (culturable count direct viable count) increased 2.5 log10 within 3 days, even after 75 days the was still 1 2 higher than inoculum size. Nutrient-depleted coccoid-shaped restored normal size assumed a bacillary shape h began divide 5 nutrient supplementation. increase decrease under nutrient-depleted conditions, as well rapid growth response supplementation, may describe some survival mechanisms V. aquatic environment.

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