Dexamethasone decreases the spermidine and spermine concentrations and polyamine oxidase activity in rat thymus.

摘要: BACKGROUND: Glucocorticoids (GCs) exert a wide range of anti-inflammatory, immunosuppressive, and antineoplastic activities. The aim our investigation was to elucidate the effect dexamethasone, synthetic GC, on polyamine metabolism in rat thymus. METHODS: Male albino Wistar rats, weighing 180-230 g, were divided into two groups: control experimental. experimental group received dexamethasone intraperitoneally for 3 days, daily dose 4 mg/animal. last hormone applied 3rd day, 1 h before killing. 0.9% NaCl instead hormone. animals killed by decapitation. thymus removed quickly rinsed with ice-cold saline. Polyamines extracted using butanol. amount polyamines investigated electrophoresis. For estimation oxidase (PAO) activity, 10% water homogenate prepared. RESULTS: Our results suggested that supplementation days significantly decreased spermine (Sp) spermidine (Spd) levels tissue (Sp Control, 362.56±25.33 nmol/g wet weight; Sp Exp. Group, 313.01±21.16 Spd 673.81±30.95 410.21±17.26 weight). PAO activity under influence comparison (PAO 0.449±0.121 U/mg prot.; 0.312±0.096 prot.). CONCLUSIONS: decrease amounts is not due change activity.