Triptolide inhibits viability and migration while promotes apoptosis in nephroblastoma cells by regulation of miR-193b-3p.

作者: Shiying Hang , Xianghong Wang , Hai Li

DOI: 10.1016/J.YEXMP.2019.04.006

关键词: TriptolideApoptosisCell biologyPI3K/AKT/mTOR pathwayMAPK/ERK pathwayViability assayWestern blotChemistryProtein kinase BKLF4

摘要: Abstract Background Triptolide (TPL) is a potential anti-tumor natural compound. However, its role in nephroblastoma poorly studied. Herein, we aimed to reveal the regulatory effects of TPL on human cells (G-401 and WiT49) as well mechanism G-401 cells. Methods Effects cell viability, migration apoptosis WiT49 were measured by CCK-8 assay, Boyden Chamber, flow cytometry/Western blot analysis, respectively. Expression miR-193b-3p TPL-treated was determined RT-qPCR. Then, whether downstream factor Alteration KLF4 expression relationship between assessed Western analysis luciferase reporter assay. abnormally expressed also assessed. Finally, involvements PI3K/AKT ERK pathways analysis. Results reduced viability while promoted level up-regulated stimulation, might function through regulation miR-193b-3p. down-regulated TPL, proven be target gene mediated repressive roles growth via knockdown. found phosphorylation PI3K, AKT inhibited possibly miR-193b-3p-mediated KLF4. Conclusion showed tumor suppressive down-regulation KLF4, along with inhibition pathways.

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