作者: Shiying Hang , Xianghong Wang , Hai Li
DOI: 10.1016/J.YEXMP.2019.04.006
关键词: Triptolide 、 Apoptosis 、 Cell biology 、 PI3K/AKT/mTOR pathway 、 MAPK/ERK pathway 、 Viability assay 、 Western blot 、 Chemistry 、 Protein kinase B 、 KLF4
摘要: Abstract Background Triptolide (TPL) is a potential anti-tumor natural compound. However, its role in nephroblastoma poorly studied. Herein, we aimed to reveal the regulatory effects of TPL on human cells (G-401 and WiT49) as well mechanism G-401 cells. Methods Effects cell viability, migration apoptosis WiT49 were measured by CCK-8 assay, Boyden Chamber, flow cytometry/Western blot analysis, respectively. Expression miR-193b-3p TPL-treated was determined RT-qPCR. Then, whether downstream factor Alteration KLF4 expression relationship between assessed Western analysis luciferase reporter assay. abnormally expressed also assessed. Finally, involvements PI3K/AKT ERK pathways analysis. Results reduced viability while promoted level up-regulated stimulation, might function through regulation miR-193b-3p. down-regulated TPL, proven be target gene mediated repressive roles growth via knockdown. found phosphorylation PI3K, AKT inhibited possibly miR-193b-3p-mediated KLF4. Conclusion showed tumor suppressive down-regulation KLF4, along with inhibition pathways.