Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions.

作者： M J Mahan , J M Slauch , J J Mekalanos

DOI: 10.1128/JB.175.21.7086-7091.1993

关键词: Transduction (genetics) 、 Biology 、 Molecular biology 、 Molecular cloning 、 Plasmid 、 Homologous recombination 、 Circular bacterial chromosome 、 Bacteriophage 、 Restriction enzyme 、 Virus Integration 、 Genetics

摘要: … phage P22 lysates grown on the donor strains were 4.8 x 1()" PFU/ml for MT1042 and 1.8 x 1()"' PFU/ml for MTI041; 10 ', 1() -2, and 1 represent serial dilutions of undiluted phage lysate. …