Overexpression of HHLA2 in human clear cell renal cell carcinoma is significantly associated with poor survival of the patients

摘要: It is well known that human clear cell renal carcinoma (ccRCC) a highly immunogenic and chemo-resistant tumor. Recently, emerging data suggest the immune checkpoint blockade therapy an important breakthrough in treatment against ccRCC. HHLA2, recently reported member of B7 family, uniquely expressed humans but not mice, it plays role functional inhibition CD4 CD8 T cells. Herein, we aimed to study clinical implications HHLA2 expression ccRCC its potential regulatory biological functions cancer In present study, examined tissues analyzed as prognostic value. The intervention lines ACHN 786-O was performed effect on cellular function cells also analyzed. We identified differentially genes upon knockdown by using gene microarray analysis. found higher mRNA level compared with adjacent normal based TCGA data, at positively significantly correlated PD-L1, PD-L2, B7-H6, negatively B7-H3. Moreover, our immunohistochemistry showed staining intensity than tissues, overall survival rate patients poorer lower expression. Higher associated larger tumor size advanced TNM stage. COX model revealed parameters including patient’s age, stage could be used independent risk factors respectively for prediction patients. Our lines, viability, migration invasion ability were inhibited, while cycle arrest G1 phase induced expressions Cyclin D1, c-Myc E1 decreased. addition, according epithelia-to-mesenchymal transition markers, such E-cadherin, N-cadherin Vimentin, changed after findings indicated involved progression predictor this malignancy.