Identification of electrophysiologically-active compounds for the malaria mosquito, Anopheles gambiae, in human sweat extracts.

摘要: Human sweat samples were chemically fractionated into acid and non-acid components. The most abundant volatile compounds present in the fractions identified by linked gas chromatography mass spectrometry. found to be composed of a range twenty aliphatic three aromatic carboxylic acids ranging, on average, from 0.02 20 micrograms per ml sampled. Non-acid contain: 6-methyl-5-hepten-2-one, 1-octen-3-ol, decanal, benzyl alcohol, dimethylsulphone, phenylethanol, phenol 4-methylphenol, collectively amounting 0.1 3 sweat. major component was L-lactic which constituted 1 5 mg/ml. Using intact antennae anthropophilic malaria vector mosquito Anopheles gambiae Giles, peripheral olfactory activities investigated electroantennography (EAG). Short-chain saturated acids, methanoic, ethanoic, propanoic, butanoic, pentanoic hexanoic elicit significantly larger EAG responses than longer chain female An.gambiae. For given dose largest amplitude response elicited methanoic acid. Pentanoic either butanoic or acids. Two non-acidic compounds, 1-octen-3-ol significant dose-dependent 1-Octen-3-ol 4-methylphenol for dose, but both smaller same C1-C6 straight-chain possible behavioural significance EAG-active human is discussed.