Widespread Gene Conversion in Centromere Cores

摘要: Centromeres are the most dynamic regions of genome, yet they typified by little or no crossing over, making it difficult to explain origin this diversity. To address question, we developed a novel CENH3 ChIP display method that maps kinetochore footprints over transposon-rich areas centromere cores. A high level polymorphism made possible map total 238 within-centromere markers using maize recombinant inbred lines. Over half were shown interact directly with kinetochores (CENH3) chromatin immunoprecipitation. Although classical is fully suppressed across domains, two gene conversion events (i.e., non-crossover marker exchanges) identified in mapping population. population genetic analysis 53 diverse inbreds suggests historical widespread centromeres, occurring at rate >1×10−5/marker/generation. We conclude accelerates evolution facilitating sequence exchange among chromosomes.