Temperature-Responsive Release of Cortisol from Its Binding Globulin: A Protein Thermocouple

摘要: Background: Only 5% of circulating cortisol is active and unbound to carrier proteins. Because levels vary rapidly due the pulsatile nature secretion, dynamics binding are critical determinants tissue free consequent hormonal signaling. The major glucocorticoid protein corticosteroid globulin (CBG), a member serpin family that undergoes conformational changes bind release hormones. This mechanism has been noted be temperature responsive, we have now investigated effects on human CBG both progesterone. Methods: Recombinant was synthesized used for studies with progesterone between 34 43 C. Binding monitored by recording change in intrinsic fluorescence. steroids other carrier, serum albumin, measured similar manner. Results: There no effect ...