Effect of Heme Oxygenase-1 on Matrix Metalloproteinase-3 Expression in Human Fibroblasts
作者： Theresa A. Stangl
DOI:
关键词: Cell culture 、 Hemin 、 Chemistry 、 Angiogenesis 、 Heme oxygenase 、 Matrix Metalloproteinase 3 、 Matrix metalloproteinase 、 COPP 、 Cancer research 、 Fibroblast
摘要: EFFECT OF HEME OXYGENASE-1 ON MATRIX METALLOPROTEINASE-3 EXPRESSION IN HUMAN FIBROBLASTS Theresa A. Stangl MS in Biomedical Sciences, July 2014 Philadelphia College of Osteopathic Medicine Ruth C. Borghaei, Thesis Advisor Heme oxygenase-1(HO-1) is an enzyme that plays a very important role the resolution inflammation. HO-1-based therapies are effective number disease conditions. However, HO-1 also increases tumor growth, angiogenesis, metastasis and chemoresistance. Matrix metalloproteinase-3 (MMP-3) involved physiological pathophysiological tissue remodeling. Unbalanced expression MMPs key feature connective destruction chronic inflammatory Previously shown this laboratory, inducer, hemin, increased MMP-3 mRNA some HGF cultures. To assess whether and/or its products regulate human fibroblasts, effect on was tested HGF, HFF, MG-63 cell lines. Cobalt protoporphyrin IX(CoPP) used to induce HO1 Tin IX(SnPP) inhibit activity. levels were quantified using real time PCR normalized GAPDH levels. Treatment fibroblast cultures (HGF, MG-63) with CoPP did not result significant changes
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