In vitro assessment of the effects of Brassica juncea and Raphanus sativus leaf and root extracts on the viability of Globodera pallida encysted eggs

摘要: The biofumigation potential of leaf and root extracts Brassica juncea Raphanus sativuson Globodera pallida were assessed in vitro. In an efficacy study, G. pallidaencysted eggs exposed to six different concentrations freeze-dried or for 96 h viability using hatching assays 6-week-old potato leachates (PRL). For B. junceaextracts LC 50value 0.027 mg ml −1w/v was determined. 50of sativusroot 0.032 −1, whereas effective only at higher (⩾0.50 −1; w/v) a lesser extent. Hatching pallidawas enhanced PRL following exposure lower (0.063 −1) R. sativusleaf extract. An analysis the types glucosinolate (GSL) present tissues revealed that juncealeaf tissue rich 2-propenyl GSL (≈98%). Root also had high concentration GSL, but found have (⩾90 μmol (g dry weight) when compared with extract (⩾10 −1). sativushad two-fold more predominantly 2-phenylethyl (⩾50 −1), which dominated by 4-methylsulfinylbutyl (⩾20 summary, strong suppression exhibited shows this species pallidamanagement if effectively incorporated into integrated cyst nematode management scheme. comparison juncea, sativuscan be improved maximising its biomass production.