The time course of malondialdehyde production following impact injury to rat spinal cord as measured by microdialysis and high pressure liquid chromatography.
作者： Hao Qian , Danxia Liu
关键词: Malondialdehyde 、 Chemistry 、 Butylated hydroxytoluene 、 High-performance liquid chromatography 、 Antioxidant 、 Chromatography 、 Spinal cord 、 Spinal cord injury 、 Central nervous system 、 Microdialysis 、 Biochemistry
摘要: This paper reports a highly sensitive, specific, and reproducible method for the analysis of malondialdehyde (MDA) from microdialysates. The microdialysates were reacted with 2-thiobarbituric acid, TBA adducts separated by HPLC detected using fluorescence detector. Butylated hydroxytoluene was used as an antioxidant to minimize formation artifacts. time course MDA production following impact injury rat spinal cord obtained this improved method. concentrations in extracellular space gradually increased basal level 20 ± 3.6 nM 44 18.1 during first 2 hr, reached maximum 95 19.8 at 5 then decreased 36 9.5 9 hr. findings support hypothesis that leads membrane lipid peroxidation.
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