Novel HPLC analysis of tocopherols, tocotrienols, and cholesterol in tissue.
作者： Eugenios Katsanidis , Paul B Addis
DOI: 10.1016/S0891-5849(99)00205-1
关键词: Vitamin E 、 Saponification 、 Lipoprotein 、 Oxidative stress 、 High-performance liquid chromatography 、 Plasma cholesterol 、 Biochemistry 、 Chemistry 、 Cholesterol 、 Hplc analysis 、 Chromatography
摘要: Tocopherols and tocotrienols are being increasingly recognized to have an important role in the prevention of atherosclerosis. It has been reported that they protect low-density lipoprotein (LDL) tissues from oxidative stress can reduce plasma cholesterol levels. Two isocratic high-performance liquid chromatography (HPLC) methods for simultaneous analysis tocopherols, tocotrienols, muscle tissue were developed. Method A involves basic saponification sample, but causes losses gamma- delta-homologs vitamin E. B does not involve saponification, thereby protecting more sensitive homologs. Both permit rapid multiple samples neither requires specialized equipment. These may provide techniques useful assessment status (OSS)
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