Methods and compositions for improving efficiency of nucleic acids amplification reactions
作者： Yan Wang , Xiao-Song Gong , Evan H. Bursey , Man Cheng , Rongdian Fu
DOI:
关键词: Nucleic acid 、 Exonuclease 、 Nucleic acid methods 、 Biology 、 Processivity 、 Polymerase 、 Molecular biology 、 Biochemistry 、 Wild type
摘要: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. encompasses hybrid polymerases that show increased processivity over wild type as well decreased exonuclease activity. also methods, kits conducting synthesis reactions in which non-specific primers is reduced.
lens.org 参考文章(71)
Panciu A, Zhao X, Haqqi T, Yadav Sp, Sequencing in the presence of betaine: Improvement in sequencing of the localized repeat sequence regions. Journal of biomolecular techniques. ,vol. 13, pp. 265- 271 ,(2002)
P. Tudzynski, Ulf Stahl, Molecular biology of filamentous fungi : proceedings of the EMBO-Workshop, Berlin, August 24-29, 1991 VCH. ,(1992)
Michael Squillace, Barry Feigenbaum, Defining hierarchical structures with markup languages and reflection ,(2004)
Paraskevi Tavladoraki, Angiola Desiderio, Eugenio Benvenuto, Rosella Franconi, Stabilizing peptides, polypeptides and antibodies which include them ,(2000)
James P. Merryweather, Lawrence S. Cousens, Patricia A. Tekamp-Olson, Jeffrey R. Shuster, Expression using fused genes providing for protein product ,(1986)
Fred Sherman, Gerald R. Fink, James B. Hicks, Methods in yeast genetics Cold Spring Harbor Laboratory. ,(1979)
Yan Wang, Sso7-Polymerase Conjugate Proteins ,(2002)
Roman Kischel, Ralf Lutterbüse, Peter Kufer, Katrin Borschert, Robert Hofmeister, Monika Mayer, Gert Riethmüller, Multifunctional polypeptides comprising a binding site to an epitope of the nkg2d receptor complex ,(2001)
Shin Yonehara, Hideyuki Haruyama, Jun Ohsumi, Masahiko Ohtsuki, Kaori Nakahara, Nobufusa Serizawa, Akio Shiraishi, Kimihisa Ichikawa, Tohru Takahashi, Hiroko Yoshida, Ikuko Tamaki, Anti-fas antibodies ,(1998)