Alcohol dehydrogenase on inorganic powders: Zeta potential and particle agglomeration as main factors determining activity during immobilization

摘要: Abstract Alcohol dehydrogenase from Saccharomyces cerevisiae was immobilized on different inorganic support materials, i.e. powders of Al2O3, SiC, TiO2 and YSZ-8, by covalent bonding physical adsorption. The raw were characterized scanning electron microscopy, BET surface area, particle size distribution ζ-potential measurements. Enzyme activity retention, storage stability recyclability evaluated the basis measured material properties. Preliminary experiments showed that buffer selection a critical factor. properties both enzyme varied considerably between buffers used; namely Tris-HCl (100 mM, pH 7) MES (40 mM, 6.5) buffers. higher more stable in buffer, whereas commonly used Tris problematic due to apparent incompatibility with formaldehyde. In MES, order decreasing covalently bonded SiC > YSZ-8 > Al2O3 > TiO2. lower performance ascribed negative material, which impeded an efficient immobilization. Particle agglomeration, caused low colloidal particles hampered systems. results this study show advantages limitations using nanoparticles as immobilization supports, highlight must be considered ensure