Sodium Thiosulfate Prevents Chondrocyte Mineralization and Reduces the Severity of Murine Osteoarthritis

摘要: Objectives Calcium-containing crystals participate in the pathogenesis of OA. Sodium thiosulfate (STS) has been shown to be an effective treatment calcification disorders such as calciphylaxis and vascular calcification. This study investigated effects mechanisms action STS a murine model OA chondrocyte Methods Hydroxyapatite (HA) crystals-stimulated chondrocytes macrophages were treated with STS. Mineralization cellular production IL-6, MCP-1 reactive oxygen species (ROS) assayed. STS's on genes involved calcification, inflammation cartilage matrix degradation studied by RT-PCR. was administered menisectomy OA, effect periarticular calcific deposits degeneration micro-CT-scan histology. Results In vitro, prevented dose-dependent manner calcium crystal deposition inhibited Annexin V gene expression. addition, there reduction crystal-induced IL-6 production. also had antioxidant effect, diminished HA-induced ROS generation abrogated catabolic responses chondrocytes. vivo, administration reduced histological severity limiting size new reducing damage. Conclusions reduces damage animal via its mineralization attenuation well enzymes generation. Our suggests that may disease-modifying drug crystal-associated