Inhibitor of DNA binding 1 (Id1) induces differentiation and proliferation of mouse embryonic carcinoma P19CL6 cells

摘要: Highlights: {yields} Id1 was upregulated during the cardiac differentiation process of P19CL6 cells. expression specific genes Gata4, {alpha}-MHC and ISL1. promoted proliferation Overexpression increased activity TOP flash. Wnt3a or LiCl treatment in -- Abstract: The inhibitor DNA binding (Id) family encodes negative regulators basic helix-loop-helix transcription factors has been implicated such diverse cellular processes as differentiation, proliferation, apoptosis migration. Id knockout mouse embryos display multiple defects but role is unclear. In present study, we investigated function DMSO-induced cells, a widely-accepted cell model differentiation. We found that marker genes, ISL1, cells stably transfected with (P19CL6-Id1) overexpression reduced number G1 phase population G2, M S phases, while knockdown from 48.6more » {+-} 2.51% to 62.2 1.52% at day 0 induction, 52.5 3.41% 63.7 1.02% 3 after indicating Luciferase assays showed flash higher P19CL6-Id1 than wildtype also treated LiCl. This indicates there may be positive feedback between Wnt signaling which plays an important differentiation.« less