Antigen presentation by supported planar membranes containing affinity-purified I-Ad.

摘要: Abstract I-Ad, purified from A20-1.11 cells by affinity chromatography, was incorporated into supported planar membranes incubation of I-Ad-containing phospholipid vesicles with clean glass coverslips. Such present a peptide digest ovalbumin to the ovalbumin-specific, I-Ad-restricted T-cell hybridoma 3DO-54.8, resulting in antigen-specific release interleukin 2. However, when same material provided form small unilamellar vesicles, no response obtained. Antigen presentation inhibited monoclonal antibody MKD6 (anti-I-Ad) but not 10-2.16 (anti-I-Ak). The GK1.5, which recognizes surface antigen L3T4, also inhibitory. In contrast results I-Ad, crude membrane preparations were effective both and vesicular forms.