N-Linked Glycosylation in Archaea: a Structural, Functional, and Genetic Analysis

摘要: N-glycosylation of proteins is one the most prevalent posttranslational modifications in nature. Accordingly, a pathway with shared commonalities found all three domains life. While excellent model systems have been developed for studying both Eukarya and Bacteria, an understanding this process Archaea was hampered until recently by lack effective molecular tools. However, within last decade, impressive advances study archaeal version important made halophiles, methanogens, thermoacidophiles, combining glycan structural information obtained mass spectrometry bioinformatic, genetic, biochemical, enzymatic data. These studies reveal features eukaryal bacterial novel archaeon-specific aspects. Unique include presence unusual dolichol lipid carriers, use variety linking sugars that connect to proteins, as constituents, two very different N-linked glycans attached same protein, ability vary N-glycan composition under growth conditions. are focus review, emphasis on pathways Haloferax, Methanococcus, Sulfolobus.