Efektivitas Pemberian Silase dan Probiotik Terhadap Ekologi Rumen Sapi Peranakan Ongol Menggunakan Analisis Terminal Restriction Fragment Length Polymorphism (T-RFLP)

摘要: RONI RIDWAN. Effectivity of Silage and Probiotic on Rumen Ecology Peranakan Ongole Cattle by Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis. Under direction SRI BUDIARTI ACHMAD DINOTO. Silages Probiotics additives were used for improvement cattle productivity. Key effectivity silage probiotics was changed observation direct or indirect roles rumen ecology. The objective this study to examine the effects ruminal ecosystems in vivo using molecular approach. Terminal-restriction fragment length polymorphism analysis detect changes ecology communities based 16S ribosomal deoxyribonucleic acid (16S rDNA). Two canulated PO fed several diet ie; (R0) basal dry matter basis (Pennisetum purpureum 70% commercial concentrate 30%), (R1) (basal fermented Lactobacillus plantarum BTCC570), (R2) + (Leuconostoc sp. BTCC531), (R3) Basal BTCC531). Digesta samples colected 3 h after feeding cellulolytic activities (DNS method), pH, parsial volatile fatty acids measurements (Gas Chromatography T-RFLP analysis. performed rDNA amplified from each sample. lengths terminal restriction fragments analysed digestion with HhaI, HaeIII MspI. Result showed that effectivities given together index Smith Wilson evenness applied data (Evar) 0.89±0.04 highest. It significantly (p<0.05) increased metabolism product (%mM) such propionic (19.44), valeric (0.76), isobutyric (2.21), isovaleric (1.77) compared diet. has a potency be comparisons complex bacterial communities, especialy community structure respons diferent variabels bacteria diversity rumen.