作者: Anthony G. Coulepis , Stephen A. Locarnini , Allan A. Ferris , Noreen I. Lehmann , Ian D. Gust
DOI: 10.1159/000148964
关键词: Hepatitis 、 Virus 、 Virology 、 Hepatitis A 、 Biology 、 Molecular mass 、 Picornaviridae 、 Differential centrifugation 、 Hepatitis B virus DNA polymerase 、 Ultracentrifuge
摘要: Hepatitis A virus was purified from fecal specimens obtained 3 patients with naturally acquired hepatitis A, by a process of differential centrifugation, chloroform extraction, column chromatography, and isopycnic ultracentrifugation. Analysis discontinuous SDS-PAGE revealed three major polypeptides molecular weights 34,000, 25,500, 23,000 daltons. These appear to be specific for have similar the four reported members genus Enterovirus within family Picornaviridae.