Simultaneous determination of carbamazepine-N-glucuronide and carbamazepine phase I metabolites in the wastewater by liquid chromatography-tandem mass spectrometry

摘要: Abstract Carbamazepine (CBZ) is one of the most frequently detected pharmaceuticals in aquatic environment. In human body, CBZ metabolized and transformed into a variety metabolites. Among metabolites, N-glucuronide (CBZ-Glu, Phase II metabolite) was discharged sewage system together with I metabolites such as 2-hydroxycarbamazepine (2-OH-CBZ), 3-hydroxycarbamazepine (3-OH-CBZ), 10-hydroxycarbamazepine (10-OH-CBZ), 10,11-Dihydroxycarbamazepine (DiOH-CBZ), carbamazepine 10,11-epoxide (EP-CBZ). However, occurrence CBZ-Glu wastewater not reported previously due to lack standard. this study, quantitative analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) developed for determination well other wastewater. method, sufficiently high extraction efficiencies its were achieved by solid phase (SPE) process (recoveries ranged from 79.3% 95.8%, 84.4% 96.4% influent effluent, respectively). The concentration effluent 59.4, 48.1 ng/L, respectively. 53.8, 61.9 ng/L, Although persistent during A2O process, deconjugation suspected contribute increase CBZ.